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. 1999 Feb 16;96(4):1433–1438. doi: 10.1073/pnas.96.4.1433

Figure 5.

Figure 5

ATF-2 and CREB activity are necessary for the activity of the cyclin D1 CRE in chondrocytes. MCT cells were cotransfected with −66CD1LUC or −66 mut CD1LUC, either empty expression vector or expression vectors for dominant-negative ATF-2 (a), dominant-negative CREB (b), or both (c), and pRlSV40. After transfection (30 h), cells were harvested, and firefly luciferase activity was measured and standardized to Renilla luciferase activity to yield the relative luciferase activity. Dominant-negative ATF-2 or CREB alone reduced the activity of −66 CD1LUC 2.5- to 3.5-fold; in combination, they reduced the activity to the levels of −66 mut CD1LUC. The activity of −66 mut CD1LUC was not affected by either dominant-negative expression vector.