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Cyclin D1 protein levels are reduced in ATF-2-deficient chondrocytes. Total protein from chondrocytes isolated from heterozygous (−/+) or homozygous (−/−) ATF-2-deficent mice were analyzed by Western blot. As expected, chondrocytes from −/− mice do not express ATF-2, in contrast to −/+ chondrocytes. The level of cyclin D1 is greatly reduced in homozygote mice. Loading of equal amounts of protein was demonstrated with an anti-actin antibody.
