TABLE 2.
Potential virulence factor expression by Bacillus spp. isolated from veterinary and clinical samples
| Bacterial species | Isolate | Toxin concn detected (ng/ml)a | % Cytotoxic activityb
|
Detection of the indicated diarrheagenic genes by PCRd
|
% of bacteria showingc:
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Normal | HT | TT | hblA | hblC | hblD | bceT | Adherence | Invasion | |||
| B. cereus | NB3 | — | 85 ± 4 | 15 | 10 ± 1 | − | − | − | − | − | − |
| NB23 | ≥128 | 77 ± 6 | 20 ± 6 | 26 ± 6 | + | + | + | + | − | − | |
| NB27 | — | 82 ± 5 | — | — | − | − | − | + | − | − | |
| NB35 | 64 | 88 ± 3 | 16 ± 1 | 24 ± 6 | + | + | + | + | − | − | |
| NB40 | ≥128 | 78 ± 3 | 18 ± 2 | 10 | + | + | + | + | − | − | |
| NB50 | ≥128 | 89 ± 1 | 22 ± 3 | — | + | + | + | + | − | − | |
| NB51 | 64 | 93 | 13 ± 1 | 11 ± 2 | + | + | + | + | − | − | |
| SU52 | ≥128 | 85 ± 2 | 12 ± 1 | 28 ± 6 | + | + | + | + | 0.03 ± 0.005 | − | |
| B. licheniformis | NB42 | — | 39 ± 5 | 9 ± 2 | — | − | − | − | − | − | − |
| NB14 | — | 66 ± 3 | 12 ± 2 | 22 ± 6 | − | − | − | − | 2.8 ± 0.22 | − | |
| B. polymyxa | NB6 | 16 | 88 ± 4 | 13 ± 1 | 18 ± 1 | + | + | + | − | − | − |
| B. coagulans | NB11 | 64 | 89 ± 6 | 10 ± 3 | 5 ± 1 | + | + | + | + | − | − |
| L. monocytogenes | SU1 | — | 82 ± 5 | 14 ± 3 | 24 ± 5 | − | − | − | − | 3.88 ± 0.37 | 1.15 ± 0.23 |
| E. coli | HB101 | — | — | — | — | − | − | − | − | − | − |
a
Detection of diarrheal enterotoxin in test media by the RPLA test. —, no diarrheal enterotoxin detected.
b
Supernatant fluids were untreated (Normal), heated (HT), or trypsin treated (TT) prior to assessment of toxicity to HEp-2 cells. Values shown are means ± standard errors for results from triplicate trials where samples were examined three times. —, no effect.
c
Values are reported as means ± standard errors. −, no adherence or invasion.
d
+, gene was detected; −, gene was not detected.