TABLE 2.
Characteristics of naphthalene-mineralizing bacterial isolates from adjacent site habitats, seep sediment and hillside soil
| Habitat/strain | Gram stain/morphologya | Oxidase | Closest taxon identified byb:
|
16S rRNA (% identity) | |
|---|---|---|---|---|---|
| API-NFT | BIOLOG | ||||
| Seep | |||||
| Cg1 | Neg./rod | + | Pseudomonas putida | Pseudomonas putida A1 | Pseudomonas gramini (100) |
| Cg2 | Neg./rod | + | Pseudomonas fluorescens | Pseudomonas fluorescens G | Pseudomonas azotoformans (99.7) |
| Cg3 | Pos./rod | − | ND | Micrococcus diversus | NDc |
| Cg4 | Neg./rod | + | No ID | No ID (Pseudomonas fulva) | Pseudomonas amygdali (99.4) |
| Cg5 | Neg./rod | + | (Pseudomonas fluorescens) | Pseudomonas fluorescens G | Pseudomonas gramini (100) |
| Cg7 | Neg./rod | + | No ID | (Pseudomonas fluorescens C) | Pseudomonas azotoformans (99.7) |
| Cg8 | Neg./rod | + | Pseudomonas fluorescens | No ID (Pseudomonas asplenii) | ND |
| Cg9 | Neg./rod | + | No ID | Pseudomonas fluorescens G | ND |
| Cg11 | Neg./rod | + | Ralstonia picketii | Pseudomonas mendocina | Pseudomonas amygdali (99.4) |
| Cg12 | Neg./rod | + | Pseudomonas fluorescens | (Pseudomonas fluorescens G) | ND |
| Cg13 | Pos./rod | − | ND | ND | ND |
| Cg14 | Pos./rod | − | ND | ND (Arthrobacter)d | ND |
| Cg15 | Neg./rod | + | Ralstonia picketii | No ID (Pseudomonas cichorii) | Pseudomonas amygdali (99.4) |
| Cg16 | Neg./rod | + | (Pseudomonas aureofaciens) | (Pseudomonas corrugata) | ND |
| Cg18 | Pos./rod | − | ND | Micrococcus diversus | ND |
| Cg20 | Pos./rod | − | ND | ND | ND |
| Cg21 | Neg./rod | − | Sphingomonas paucimobilis | Sphingobacterium mizutaii | Pseudomonas azotoformans (100) |
| Hillside | |||||
| Hg1 | Neg./rod | − | No ID | (Chryseomonas luteola) | Herbaspirillum seropedicae (96.6) |
| Hg2 | Neg./rod | − | No ID | (Burkholderia glathei) | Burkholderia glatheii (97.7) |
| Hg3 | Neg./rod | − | No ID | Burkholderia glathei | Burkholderia glatheii (97.8) |
| Hg4 | Neg./rod | − | No ID | Burkholderia glathei | Burkholderia glatheii (97.9) |
| Hg5 | Neg./rod | − | No ID | Burkholderia glathei | Burkholderia glatheii (98.0) |
| Hg6 | Neg./rod | − | Burkholderia cepacia | (Burkholderia glathei) | ND |
| Hg7 | Neg./coccus | − | No ID | (Burkholderia glathei) | Burkholderia glatheii (97.3) |
| Hg8 | Neg./rod | − | Burkholderia cepacia | Burkholderia gladioli | Burkholderia phenazinium (97.7) |
| Hg9 | Neg./rod | − | No ID | Burkholderia sp. (B. gladioli) | ND |
| Hg10 | Neg./rod | − | Burkholderia cepacia | Burkholderia gladioli | Burkholderia phenazinium (97.8) |
| Hg11 | Neg./rod | − | No ID | No ID (B. glathei) | Burkholderia glathei (98.1) |
| Hg12 | Neg./rod | − | Burkholderia cepacia | No ID (B. gladioli) | ND |
| Hg13 | Neg./rod | − | No ID | Burkholderia sp. (B. gladioli) | ND |
| Hg14 | Neg./rod | − | No ID | (Pseudomonas fluorescens G) | Burkholderia phenazinium (97.9) |
| Hg15 | Neg./rod | − | No ID | Burkholderia glathei | ND |
| Hg16 | Neg./rod | − | No ID | Burkholderia glathei | Burkholderia phenazinium (97.5) |
| Hg17 | Neg./rod | − | Burkholderia cepacia | Burkholderia gladtoli | Burkholderia glathei (98.1) |
| Hg18 | Neg./rod | + | No ID | Burkholderia glathei | Burkholderia glathei (98.0) |
| Hg19 | Neg./rod | − | No ID | No ID (B. glathei) | Burkholderia glathei (98.1) |
| Hg20 | Neg./rod | − | No ID | Xanthomonas maltophilia | ND |
| Hg21 | Neg./rod | − | No ID | No ID (Burkholderia glathei) | ND |
| Hg22 | Neg./rod | − | Burkholderia cepacia | Burkholderia gladioli | ND |
a
Neg., negative; Pos., positive.
b
Names in parentheses indicate the database taxon closest to the unidentified genus or species. ND, not determined; ID, identification. The API-NFT test kit is designed for gram-negative bacteria only.
c
ND, not done
d
Based on Biolog characterization and microscopic observation of coccus/irregular rod cycling