TABLE 4.
Strain | Potential metabolite detecteda
|
% of 1,2-DHDN labeled with the indicated no. of 18O atomsb
|
|||||||
---|---|---|---|---|---|---|---|---|---|
Catechol | Gentisate | Salicylate | Salicylald. | 1,2-DHN | 1,2-DHDN | 0c | 1d | 2e | |
P. putida G7 | + | BD | + | + | + | + | 66 | 11 | 22 |
C. testosteroni GZ42 | BD | + | + | + | + | + | — | ||
Hg1 | BD | BD | + | BD | + | + | 46 | 9 | 45 |
Hg2 | + | BD | + | BD | + | + | 55 | 9 | 36 |
Hg3 | + | BD | + | BD | + | + | — | ||
Hg4 | + | BD | + | + | + | + | 45 | 9 | 46 |
Hg6 | + | BD | + | + | + | + | — | ||
Hg8 | + | BD | + | + | + | + | 55 | 10 | 35 |
Hg11 | BD | + | + | + | + | + | — | ||
Hg12 | + | BD | + | BD | + | + | 62 | 10 | 28 |
+, detection; BD, below detection. Salicylald., salicyladehyde; 1,2-DHN, 1,2-dihydroxynaphthalene.
Mass spectrometry determined the distribution of 18O within 1,2-DHDN parent ions produced under an atmosphere of 50% 18O2-50% 16O2. —, not determined.
1,2-DHDN mass contained two 16O atoms. Molecular ion = M.
1,2-DHDN mass contained one 16O atoms and one 18O atom. Molecular ion = M + 2.
1,2-DHDN mass contained zero 16O atoms and two 18O atoms. Molecular ion = M + 4.