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MBCs for biofilm and free-swimming PAO1 cells in response to copper treatment in MOPSO-buffered saline. Biofilm and free-swimming cultures were grown in MSVP at room temperature by using the rotating-disk biofilm reactor. Samples of biofilm and free-swimming cells were harvested and then subjected to a range of Cu concentrations in MOPSO-buffered saline at 37°C for 5 h. Symbols: •, biofilm; ○, free-swimming cells.
