Skip to main content
. 1999 Feb 16;96(4):1445–1450. doi: 10.1073/pnas.96.4.1445

Figure 3.

Figure 3

Identification of ks1 promoter elements as specific binding sites for nuclear protein from apical (head tissue and TFZ) or basal (gastric) tissue. (A–D) Examples of two sequence elements that are related to known transcription-factor binding sites are shown. (E–H) Two examples for elements not related to known transcription-factor binding sites are shown. C, control (without protein); A, nuclear extract from apical tissue; B, nuclear extract from basal tissue; AG/G, sequencing reaction; cu, addition of unspecific competitor; cs, addition of specific competitor; ∠, rising concentrations of unspecific or specific competitior (10- or 100-fold molar excess); FP, free probe; ∗, binding of general nuclear protein(s) to oligonucleotide; I–IV, specific oligonucleotide/protein complexes. Boxes indicate DNaseI-resistant and/or -hypersensitive regions in footprint experiments. Sequences of oligonucleotides used are: kruppel-like, 5′-GTAAAAATCGGGTTTATTCG-3′; Oct-1-like, 5′-GTGCCAAAGAATGCAAAGAAAAGTTCTA-3′; Hyko-1, 5′-TTCAAAAAAACCTCAAAGATAAAAGAAGCAAAC-3′; Hyko-2, 5′-GTTTTTTGTAGCTCTACTTTTTAAAGCCAAGGTAGA-3′.