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Supershift experiments with the 42-bp dfd-1 element and Cnox-2 antiserum (gift from H. Bode, University of California, Irvine, CA). A, nuclear extract from apical tissue; B, nuclear extract from basal tissue; FP, free probe; ∗, unspecific binding to oligonucleotide; I–IV, dfd-1–protein complexes using nuclear extract from apical and basal tissue; SA, supershift obtained by using nuclear extract from apical tissue; SB, supershift obtained by using nuclear extract from basal tissue.
