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. 2003 Apr;69(4):1959–1966. doi: 10.1128/AEM.69.4.1959-1966.2003

FIG. 4.

FIG. 4.

Effects of SLDH disruption on keto-d-gluconates, l-sorbose and d-xylulose production with the resting cells of G. suboxydans IFO 3257 at acidic and alkaline pHs. G. suboxydans IFO 3257 wild-type strain and SLDH-defective mutant 3257sldA::Km were cultivated for 24 h on a glycerol medium and washed with buffer to prepare the resting cells, as described in Materials and Methods. The resting cell reaction was performed for 3 h at 30°C in a reaction mixture consisting of 100 μl of 1 M substrate, 100 μl of the resting cell suspension of 3257 (protein contents, 14.5 mg/ml [•]) and of 3257sldA::Km (13.8 mg/ml [○]), and 800 μl of buffer (pH 3.0 to 8.0; McB, pH 9.0 to 10.0; 0.2 M glycine-NaOH buffer). 2KGA (A) and 5KGA (B) concentrations were measured enzymatically while l-sorbose (C) and d-xylulose (D) concentrations were measured by the resorcinol reagent as described in Materials and Methods.