TABLE 1.
Effects of SLDH disruption on polyol dehydrogenase activities in both acidic and alkaline pHsa
| Substrate | Polyol dehydrogenase activity (U/mg) at:
|
|||
|---|---|---|---|---|
| pH 5.0
|
pH 9.0
|
|||
| Wild strain | 3257sldA::Km | Wild strain | 3257sldA::Km | |
| d-Arabitol | 1.94 | NDb | 0.96 | 0.04 |
| d-Sorbitol | 1.64 | ND | 0.75 | 0.04 |
| d-Mannitol | 1.12 | ND | 0.46 | ND |
| Ribitol | 1.17 | ND | 0.62 | 0.13 |
| meso-Erythritol | 1.56 | ND | 0.97 | ND |
| Glycerol | 1.38 | ND | 0.92 | ND |
| d-Gluconate | 0.73 | 0.12 | 0.06 | ND |
| d-Glucose | 0.99 | 0.68 | —c | — |
| Acetaldehyde | 1.99 | 2.37 | — | — |
a
G. suboxydans IFO 3257 and 3257sldA::Km were cultivated for 24 h on a glycerol medium, and the membrane fractions were prepared from these cells. Enzyme activity was measured by PMS-DCIP assay in the presence of 100 mM substrate in McB, pH 5.0, and in 0.2 M glycine-NaOH buffer, pH 9.0.
b
ND, not detected.
c
—, not determined.