TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| Sinorhizobium sp. strains | ||
| BR816 | Broad-host-range Sinorhizobium strain isolated from Leucaena leucocephala | 16 |
| FAJ1600 | cysD mutant of BR816; Tcr | This study |
| FAJ1604 | nodP1 nodQ2 cysD triple mutant of BR816; Kmr Spr Tcr | This study |
| CFNE205 | nodP1 mutant of BR816; Kmr | 25 |
| CFNE206 | nodQ2 deletion mutant of BR816; Spr | 25 |
| CFNE207 | nodP1 nodP2 double mutant of BR816; Kmr Spr | 25 |
| CFNE208 | nodP1 nodQ2 double mutant of BR816; Kmr Spr | T. Laeremans, unpublished results |
| Plasmids | ||
| pBRE4.8 | pUC19 carrying the BR816 cysDN genes; Apr | This study |
| pJQ200uc1 | B. subtilis sacB-containing suicide vector; Gmr | 39 |
| pHP45Ω-Tc | Vector containing Tcr cassette | 38 |
| pUC18/19 | Cloning vector; Apr | 33 |