FIG. 2.

Western blot analysis of the SlpA adhesion-mediating region. Total cellular proteins and cell wall-associated polypeptides were extracted from L. lactis NZ9000 cells harboring pKTH5056. The protein samples were prepared from equal amounts of cells as described in Materials and Methods, and samples were separated by SDS-PAGE, electroblotted onto a nitrocellulose membrane, and incubated with polyclonal SlpA antiserum diluted 1:1,000. Lanes: 1, total cellular proteins from nisin-induced recombinant cells; 2, cell wall-associated polypeptides from nisin-induced recombinant cells; 3, cell wall-associated polypeptides from noninduced recombinant cells. Sizes of molecular mass marker proteins are indicated on the left.