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Immunofluorescence microscopy of recombinant L. lactis NZ9000 cells harboring pKTH5056 (A) and wild-type L. lactis NZ9000 (B). Recombinant cells were harvested after overnight nisin induction, and wild-type cells were harvested after overnight incubation. Both cell types were treated with anti-SlpA antibodies (diluted 1:20 in PBS) and a fluorescein isothiocyanate-conjugated secondary antibody. Both pictures were taken after 16-s exposures. Magnification, ×4,300.
