Effect of australifungin on the growth of LCB3 synthetically lethal mutants. LCB3 synthetically lethal mutants E37, U48, U4, U33, U63, and U9 were tested for australifungin sensitivity in a broth microdilution assay and compared to the screening strain Y388-2p. The OD600 was observed after 48 h of growth in the presence of drug.