FIG. 2.

idi gene expression under starvation conditions. A wild-type, non-self-incompatible, strain was grown on synthetic SA medium for 40 h and then transferred for 4 h onto fresh SA medium to rule out any preexisting starvation. Cultures were then transferred onto SA medium lacking dextrin (-C), SA medium lacking ammonium acetate (-N), or control SA medium (cont.). Total RNAs were extracted after 1, 2, or 4 h. Total RNA samples (30 μg per lane) were analyzed by Northern blotting and probed successively with the different idi genes and a control 28S rDNA probe.