FIG. 7.

Cytological evidences of induction of autophagy by rapamycin: relocalization of the GFP-IDI7 marker and detection of autophagic bodies. A gfp-idi7 strain (A) and a gfp-idi7 ΔpspA strain (B) were grown for 16 h on synthetic SA medium. The strains were then transferred on fresh SA medium (− rapamycin) or SA medium containing rapamycin at 200 ng · ml⁻¹ (+ rapamycin). The strains were further incubated for 1 h at 26°C before observation by light microscopy (top) and by fluorescence microscopy (bottom) (scale bar = 3 μm). Note the granular aspect of the vacuoles of the ΔpspA strain in the presence of rapamycin.