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. 2003 Apr;2(2):328–340. doi: 10.1128/EC.2.2.328-340.2003

FIG. 6.

FIG. 6.

PCR analysis of the MTL loci of C. glabrata. (A) PCR assays were performed with genomic DNAs of 10 C. glabrata strains. Five separate PCRs were performed with each strain to test the presence or absence of the full-length ORFs. Note that the primers CGPF1 and P1RACE1 do not discriminate between MTLa2 and MTLα2, and the primers CGPIR2 and PIRACE1 generate a fragment containing MTLα1, MTLα2 and the intergenic promoter region. (B) Schematic representation of MTL2a, MTL1a, and MTL3α from strain 7549 and MTL1α from strains PB621 and 1480.47. Positions of key primer pairs used in the PCR analysis are shown.