FIG. 7.

Northern analysis of the expression of MTLα1 and MTLa1 in 12 strains of C. glabrata. Approximately 20 μg of total cellular RNA was applied to each lane. Duplicate Northern blots were probed with either the 300-bp PCR fragment (primers CGAR1 and A1RACE1) containing the MTL1a1 ORF or the 550-bp PCR fragment (primers CGPIF1 and CGPIR2) containing the MTL1α1 ORF. To assess loading, ethidium bromide-stained 28S rRNA patterns are included. The approximate molecular sizes of transcripts are shown to the right of the hybridization patterns. The class and MTL1 expression pattern of each strain are noted.