FIG. 8.

Northern analysis of the expression of MTLα1 and MTLa1 in the switch phenotypes of an MTLα-expressing strain (1480.49) and an MTLa-expressing strain (35B11). Cells of the two strains were plated on agar containing 1 mM CuSO₄, which discriminates between the switch phenotype in the core switching system of C. glabrata (26, 27). Cells from Wh, LB, and DB colonies of strain 1480.49 and from IWr, LB, and DB colonies of strain 35B11 were picked and replated before they were analyzed. Duplicate blots were probed with either the 550-bp PCR fragment (primers CGPIF1 and CGPIR1) containing the MTL1α1 ORF or the 300-bp PCR fragment (primers CGAR1 and A1RACE1) containing the MTL1a1 ORF. To assess loading, ethidium bromide-stained 28S rRNA patterns are included. The class and MTL1 expression pattern of each strain are noted.