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. 2003 Apr;2(2):256–264. doi: 10.1128/EC.2.2.256-264.2003

FIG. 1.

FIG. 1.

Transcript profiling reveals that UK-118005 treatment induces a GCN4 response. (A and B) Transcript profiling of cultures of MMB1489 (GCN4+) (A) and MMB1576 (gcn4Δ) (B). In each panel, normalized intensity values from untreated cultures (x axis) are compared to treated samples (y axis). Only genes whose normalized intensity is >0.1 are shown, since this value represents the approximate intensity at which the signal-to-noise ratio decreases sharply. (A) A set of known Gcn4p target genes (blue squares) (28) involved in amino acid biosynthesis is significantly upregulated in UK-118005-treated (3 μg/ml) cells. These target genes comprise the bulk of the highly induced genes in this data set. (B) In gcn4Δ cells, a similar treatment (6 μg/ml) produces few highly induced genes. For comparison, the set of genes encoding the subunits of the 26S proteasome (red triangles) (12) are unchanged by the treatment in either genetic background. For the relevance of this finding, please refer to Fig. 8.