FIG. 2.

(A) Schematic map of the plasmids used to examine the regions of the 5′ upstream 470-bp segment, which may be responsible for silencing. Using the primers described in Table 1, plasmids psAP-2 to psAP7 were derived from plasmid psAP-1. (B) Northern blots probed with ap-a gene. (C) Northern blot probed with the ribosomal protein gene EhRP-L21. (D) Western blot reacted with anti-AP-A antibodies and horseradish peroxidase anti-rabbit antibodies. Lane HM-1, control parent strain; lane 1, psAP-1; lane 2, psAP-2; lane 3, psAP-3; lane 4, psAP-4; lane 5, psAP-5; lane 6, psAP-6; lane 7, psAP-7.