FIG. 6.

Hybridization of blots with radiolabeled nascent RNA extracted from nuclear run-on experiments of control nontransfected HM-1:IMSS trophozoites and AP-A-lacking trophozoites of clone G3. E. histolytica DNA samples on the blots were prepared by PCR on E. histolytica DNA and included the ribosomal protein L21 (RP-L21), the ap-a gene (AP-A), a 5′ flanking fragment (120 bp) of the ap-a gene (bp −470 to −350) [5′ AP(Dist.120)], actin, genomic DNA (gDNA), and DNA of tandemly linked ap-b and ap-c genes (AP-B/C). 300 ng of each DNA was spotted and denatured prior to hybridization.