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Growth of gef1 and nhx1 mutants in the presence of toxic cations. Five-fold serial dilutions (starting at 105 cells) of the indicated strains were grown at 30°C for 2 days on YPD (1% yeast extract/2% peptone/2% dextrose) with the addition of either 3 mM MnCl2, 0.45 M tetramethylammonium (TMA), or 0.05 mg/ml hygromycin B (HYG) as indicated.
