Figure 5.
Expression of A. thaliana NHX1 in nhx1 yeast mutants. (A) Vector pAD4 (14) was introduced into wild-type and nhx1 strains. Plasmid pRG308; ADH: AtNHX1 was introduced into nhx1 mutant as indicated. Five-fold serial dilutions (starting at 105 cells) of the indicated strains were grown at 30°C for 2 days on YPD (−) or on YPD supplemented with 0.05 mg/ml hygromycin (+). Serial dilutions of the same strains were grown on APG medium (see Materials and Methods) (−) or on APG supplemented with 0.4 M NaCl (+) (12).