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. 2003 May;10(3):459–468. doi: 10.1128/CDLI.10.3.459-468.2003

FIG. 1.

FIG. 1.

PCR amplification of the ORF encoding the P46 and P65 membranous proteins of M. hyopneumoniae (strain ATCC 25934). Lanes 1 and 2 contain 1,260- and 1,803-bp DNA amplicons corresponding to the entire P46 and P65 gene, which were obtained by PCR using the oligonucleotide primer pairs P46BamH1-P46Sal1 and FSLP65-R2SP65, respectively. As controls M. hyorhinis and A. laidlawii were used, respectively. Lanes 3 and 4 contain P46 primers; lanes 5 and 6 contain P65 primers. Lane M, molecular sizes of fragments of the 1-kb Plus DNA ladder.