TABLE 2.
Pig inoculated (immunization) |
A450 determined with recombinant protein on:
|
|||||||
---|---|---|---|---|---|---|---|---|
D0
|
PID 14
|
PID 21
|
PID 42
|
|||||
P46 | P65c | P46 | P65c | P46 | P65c | P46 | P65c | |
55 (GST − P46) | 0 | 0 | 0.5 | 0 | 0.6 | 0 | 1.6 | 0 |
52 (GST − P46) | 0 | 0 | 0.8 | 0 | 0.6 | 0 | 1.4 | 0.5 |
53 (GST − P65c) | 0 | 0 | 0 | 1.5 | 0 | 1.0 | 0 | 2.0 |
57 (GST − P65c) | 0 | 0 | 0 | 0.5 | 0 | 1.0 | 0 | 1.2 |
56 (P46+P65c) | 0 | 0 | 0.5 | 0 | 0.95 | 0 | 2.0 | 1.5 |
58 (P46+P65c) | 0 | 0 | 0.5 | 0 | 1.0 | 0 | 0.9 | 0.8 |
Control 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Control 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Pigs were immunized by injection of individual or mixed P46 or P65c recombinant proteins, and A450 was determined by ELISA using either recombinant protein as the antigen.