FIG. 1.

Real-time PCR analysis of IEX-1 transcripts accumulating in HSV-1-infected cells. Total RNA was extracted at the indicated hours after infection from HSV-1(F)-infected HFF (A) or HeLa (B) cells and was analyzed by real-time PCR. The amount of total RNA was normalized with respect to reverse-transcribed 18S rRNA. The amount of IEX-1 RNA accumulating in infected cells was calculated as the fold change compared to that from cells harvested 1 h after mock infection.