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. 2003 Jun;77(11):6178–6187. doi: 10.1128/JVI.77.11.6178-6187.2003

FIG. 8.

FIG. 8.

Effect of IEX-1 overexpression on HSV-1 replication. (A) SK-N-SH cells were mock infected (lanes 1, 2, 6, and 7) or were infected with 10 PFU of wild-type baculovirus (Bac-WT; lanes 3 and 8), IEX-1 baculovirus (Bac-IEX-1; lanes 4 and 9), or anti-IEX-1 baculovirus (Bac-anti-IEX-1; lanes 5 and 10) per cell, respectively. After 16 h of incubation the cells were exposed to 1 PFU of HSV-1(F)/cell (lanes 2 to 5 and 7 to 10). Cells were collected at 6 (lanes 1 to 5) and 12 h (lanes 6 to 10) after exposure to HSV-1(F). Protein extracts were electrophoretically separated on a 12.5% denaturing polyacrylamide gel, electrically transferred to a nitrocellulose sheet, and reacted with the indicated antibodies as described in Materials and Methods. (B) IEX-1 protein detection. Protein extracts from SK-N-SH cells infected with IEX-1 baculovirus and exposed to HSV-1 for 6 (lane 1) or 12 h (lane 2) or only exposed to HSV-1 for 6 h (lane 3) were electrophoretically separated on a 12.5% denaturing polyacrylamide gel, electrically transferred to a nitrocellulose sheet, and reacted with a goat anti-IEX-1 polyclonal antibody. inf, infection.