TABLE 1.
Oligonucleotides used for amplification of segments of the HPV-16 genome
| Amplicona | Primer | Position | Sequence |
|---|---|---|---|
| G1 | G1F | 515-534 | GTCTTGTTGCAGATCATCAAGA |
| G1R | 1528-1509 | ATTCTGAAAAACTCACCCCG | |
| G2 | G2F | 1501-1520 | GAGTTATACGGGGTGAGTTT |
| G2R | 2526-2505 | CAATGGTCTATGCTTTACATCC | |
| G3 | G3F | 2501-2522 | CTATGGATGTAAAGCATAGACC |
| G3R | 3501-3481 | TTTCCGGTGTCTGGCTCTGAT | |
| G4 | G4F | 3471-3490 | AGCGACCAAGATCAGAGCCA |
| G4R | 4472-4453 | GGCCTTGTTCCCAATGGAAT | |
| G5 | G5F | 4402-4421 | GGTGGGTTAGGAATTGGAAC |
| G5R | 5402-5383 | GATGTAGAGGGTACAGATGG | |
| G6 | G6F | 5367-5386 | TTCTACAACCCCGGTACCAT |
| G6R | 6370-6351 | GTCGCCATATGGTTCTGACA | |
| G7 | G7F | 6348-6367 | TGGTGTCAGAACCATATGGC |
| G7R | 7173-7150 | CAACATACATACAATACTTACAGC | |
| G8 | G8F | 7145-7184 | CGTAAGCTGTAAGTATTGTATGTATGTTGAATTAGTGTTG |
| G8R | 559-528 | TTACAGCTGGGTTTCTCTACGTGTTCTTGATG | |
| P2 | P1F | 7145-7184 | CGTAAGCTGTAAGTATTGTATGTATGTTGAATTAGTGTTG |
| P2R | 122-94 | CCTGTGGGTCCTGAAACATTGCAGTTCT | |
| P4 | P4F | 7851-7886 | TGTGTGCAAACCGTTTTGGGTTACACATTTACAAG |
| P1R | 559-528 | TTACAGCTGGGTTTCTCTACGTGTTCTTGATG | |
| P5 | P5F | 99-132 | ATGCACCAAAAGAGAACTGCAATGTTTCAGGAC |
| P5R | 559-528 | TTACAGCTGGGTTTCTCTACGTGTTCTTGATG | |
| P11 | P11F | 1-38 | ACTACAATAATTCATGTATAAAACTAAGGGCGTAACCG |
| P2R | 122-94 | CCTGTGGGTCCTGAAACATTGCAGTTCT |
a
Amplicons G1 to G8 overlap and cover comprehensively the whole HPV-16 genome. Amplicons P2, P4, P5, and P11 were used to study specifically the enhancer-promoter-E6 segment.