TABLE 3.

Methylation of 11 CpGs in the LCR of HPV-16 in two cell lines and 15 clinical samples

Cell line or samplea	Methylation of CpG at positionb:
	7535	7554	7677	7683	7695	7862	31	37	43	52	58
SiHa	−	−	−	−	−	−	−	−	−	−	−
CaSki	+	+	+	+	+	+	+	+	+	+	+
As16	+	+	+	+	+	+	+	+	+	+	+
As22	+	+	+	+	+	+	+	+	+	+	+
As23	+	+	+	+	+	+	+	+	+	+	+
As24	+	+	+	+	+	+	+	+	+	+	+
As33	+	+	+	+	+	+	+	+	+	+	+
CIN I A1	+	+	+	+	+	−	+	−	−	−	−
CIN I A6	+	+	−	−	−	−	+	+	−	−	−
CIN III A20	+	+	+	+	+	−	+	+	+	+	+
T6	+	+	+	+	+	−	+	+	+	+	+
T8	+	±	−	+	−	−	−	−	+	+	+
T9	?	+	+	±	±	±	+	+	+	+	+
T10	+	+	+	+	+	−	+	+	+	+	+
T20	?	?	+	±	±	±	+	+	+	+	+
T37	−	−	−	−	−	−	+	+	+	+	+
T39	+	+	+	+	+	−	−	−	−	−	−

^aThe five CpGs between positions 7535 and 7695 are close to cis-responsive elements of the viral enhancer; the CpGs between positions 31 and 58 overlap with promoter elements; and the CpG at position 7862 is located between the borders of two specifically positioned nucleosomes, one of which overlaps the enhancer while the other overlaps the promoter.

^b+, methylation; −, lack of methylation; ±, both methylated and unmethylated residues in the same sample; ?, unknown.