Figure 4.

(A) A color photograph of a chicken muscle tissue section in which the ryanodine receptors have been immunogold labeled, and then silver enhanced until individual PRPs have been formed. The optical microscope configuration and detection system is similar to that used for the data of Fig. 2. The white arrows indicate the direction of the parallel set of z lines located between the lines of PRPs. (Bar = 2 μm.) (B) An intensity (count) line scan along the direction indicated by the single arrow in A, demonstrating that the individual PRP image peaks are readily resolved. (C) A transmission electron micrograph of a tissue section similar to that used for A, confirming that the PRPs are found along the z lines in a latticelike fashion characteristic of the known spatial distribution of ryanodine receptors at the sarcoplasmic reticulum and transverse tubule system junctions. (Bar = 1 μm.)