Figure 5.

Results of a PRP-based immunoassay. In this test assay, a polystyrene surface coated with biotinylated BSA served as the reaction substrate. After blocking this surface with nonbiotinylated BSA, a dilution series of GAB antibody (constituting the target), in amounts ranging from 0 (control) to 600 pg, was added and allowed to incubate overnight. After a wash step, PRPs conjugated with RAG antibodies were then incubated with the samples for 1 h, after which the samples were washed, air dried, and imaged.