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TMEV-induced cytokine expression in astrocytes is inhibited by NF-κB inhibitors. Various concentrations (0.01 to 10 μM) of CAPE (NF-κB inhibitor) and MG-132 (proteasome inhibitor) were added 3 h prior to TMEV infection to determine its specific role in cytokine gene expression. Cytokine expression in control and experimental astrocyte cultures was analyzed at 6 h postinfection (MOI = 10) by RPA using two separate murine cytokine multiprobe sets (Pharmingen).
