FIG. 3.

Intracellular colocalization of UL6AU1 and UL28. Vero cells were transfected with pAS30AU1 expressing UL6AU1 (panels a to c), pUL28 expressing UL28 (panels d to f), or both plasmids together (panels g to i). The proteins were detected by an indirect immunofluorescence assay with the AU1 mouse monoclonal antibody, specific for UL6AU1, and the R123 polyclonal rabbit antibody, specific for UL28, as primary antibodies and Cy5-conjugated anti-mouse IgG and FITC-conjugated anti-rabbit IgG as secondary antibodies. The cells were examined by confocal microscopy. Each set of three digital confocal images (from left to right) shows the same field of cells, with FITC fluorescence in green, Cy5 fluorescence in red, and a merged image of the two.