TABLE 1.
Complementation of UL6 null mutant by HSV-1 UL6 insertional mutants
| Plasmid | UL6 amino acid change (from/to) | Localizationb | Virus titer (PFU/ml)c |
|---|---|---|---|
| pCMV10 | <20 | ||
| pAS30 | Nuclear | 1.0 × 106 | |
| pUL6in161 | 161I-162D/I-ARSR-D | Cytoplasmic | <10 |
| pUL6in269 | 269F-D270/F-ARSR-D | Cytoplasmic | <10 |
| pUL6in368 | 368A-369G/A-ARSR-G | Nuclear | <10 |
| pUL6in378 | 378V-379D/DV-ARSR-D | Nuclear | 8.6 × 106 |
| pUL6in379Ea | 379D-380G/D-TYRYI-G | Nuclear | 8.8 × 106 |
a
This plasmid contains the coding sequences for the AU1 epitope (amino acids TYRYI) inserted into the UL6 ORF.
b
The distribution of the transiently expressed protein in Vero cells was determined by using an indirect immunofluorescence assay.
c
Vero cells were transfected with plasmid DNA and subsequently infected with the UL6 null mutant. The virus yield was determined on the complementing cell line.