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Complementation of the UL28 null mutant by HSV-1 UL28c-myc
| Plasmid | UL28 amino acid change (from/to) | Virus titer (PFU/ml)a |
|---|---|---|
| pCMV10 | <10 | |
| pUL28 | 3.0 × 104 | |
| pUL28-c-myc | 465G-GGGEDEDRRRG-P/ G-EQKLISEEDL-Pb | 1.5 × 104 |
Vero cells were transfected with plasmid DNA and subsequently infected with the UL28 null mutant. The virus yield was determined on the complementing cell line.
Amino acids 465 to 477 were replaced by 10 amino acids specifying c-myc epitope.
