TABLE 3.

Susceptibility of cells derived from calf tissues to CAEV

Cell origin	Passage no. and CAEV detection results for calfa:
	NI-3				I-1				I-2
	Passage no.	CPE in culture	CPE in coculture	Infectivity	Passage no.	CPE in culture	CPE in coculture	Infectivity	Passage no.	CPE in culture	CPE in coculture	Infectivity
Spleen	ND	ND	ND	ND	6	+++	+++	+	2	+++	+++	+
Median lobe	4	+++	+++	+	5	+	++	+	3	+++	+++	+
Thymus	2	+++	+++	+	ND	ND	ND	ND	ND	ND	ND	ND
Choroid plexus	3	+	+	+	5	+	+	+	3	+	+	+
Bone marrow	4	+++	+++	+	5	+++	+++	+	2	+++	+++	+
Synovial membrane	3	++	++	+	5	+++	+++	+	2	+++	+++	+

^aCalves NI-3, I-1, and I-2 were autopsied at 6, 7, and 24 months of age, respectively. Explant cultures were derived from various tissues and were serially passaged before CAEV inoculation at the indicated passage numbers. Cytopathic effects in cultures, or cocultures with the indicators GSM cells and the presence of infectious cytopathic virus in the supernatants of infected explant cultures were examined. (+, presence of CPE or infectious virus; ND, not determined).