FIG. 1.
Appearance of the GP2b, GP3, and GP4 proteins in EAV particles. EAV-infected BHK-21 cells were labeled with l-[35S]cysteine from 6.5 to 11 h p.i. After removal of cell debris from the cell culture medium by low-speed centrifugation, the extracellular virions were pelleted through a cushion of 20% (wt/wt) sucrose. The pellet was dissolved in lysis buffer, and immunoprecipitations (IP) with αGP2b (2b), αGP3 (3), or αGP4 (4) in the presence or absence of 5 mM DTT were carried out. The samples were finally dissolved in LSB with or without 50 mM DTT and analyzed in SDS-15% PAA gels. The numbers on the right are the molecular masses, in kilodaltons, of marker proteins analyzed in the same gel. Ab, antibody.