FIG. 3.
Presence of covalently linked GP2b/GP3/GP4 trimers in EAV-infected cells. EAV-infected BHK-21 cells were pulse-labeled for 15 min with l-[35S]cysteine starting at 8.25 h p.i. and chased for the indicated time periods (in minutes). Next, cell lysates were prepared and immunoprecipitations were performed with αGP2b (2b) or with the corresponding preimmune serum (p2b). The immunoprecipitates were analyzed under nonreducing conditions in SDS-15% PAA gels. As a reference, the immune complexes obtained after incubation of an aliquot of solubilized EAV particles with αGP2b or the corresponding preimmune serum were run in parallel (lanes V). The numbers on the left are the molecular masses, in kilodaltons, of marker proteins analyzed in the same gel. Ab, antibody.