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Effect of the oxidizing agent diamide on the conversion of disulfide-bonded GP2b/GP4 dimers into covalently linked GP2b/GP3/GP4 trimers. The culture supernatant of [35S]cysteine-labeled EAV-infected cells was split into equal fractions and incubated in the presence of different concentrations of diamide for 1 min at 4°C or RT. The samples were subsequently processed as described in the legend to Fig. 4. The numbers on the left are molecular masses in kilodaltons.
