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. 2003 Jun;77(11):6438–6449. doi: 10.1128/JVI.77.11.6438-6449.2003

TABLE 1.

Synthetic oligonucleotides used for the generation of His-VP3 carboxy-terminal end deletion mutantsa

3′ primer Sequence
His-VP3Δ248-257 CGC GGG TAC CTT ACC AGC GGC CCA GCC GAC C
His-VP3Δ243-257 CGC GGG TAC CTT AAC CAG GGG GTC TCT GTG TTG
His-VP3Δ238-257 CGC GGG TAC CTT ATG TTG GAG CAT TGG GTT TTG
His-VP3Δ233-257 CGC GGG TAC CTT ATT TTG GCT TGG GCT TTG G
His-VP3Δ228-257 CGC GGG TAC CTT ATG GTA GAG CCC GCC TGG G
a

The 5′ primer was GGG GGA ATT CAT GGC ATC AGA GTT CAA AGA GAC CCC C.