TABLE 1.
3′ primer | Sequence |
---|---|
His-VP3Δ248-257 | CGC GGG TAC CTT ACC AGC GGC CCA GCC GAC C |
His-VP3Δ243-257 | CGC GGG TAC CTT AAC CAG GGG GTC TCT GTG TTG |
His-VP3Δ238-257 | CGC GGG TAC CTT ATG TTG GAG CAT TGG GTT TTG |
His-VP3Δ233-257 | CGC GGG TAC CTT ATT TTG GCT TGG GCT TTG G |
His-VP3Δ228-257 | CGC GGG TAC CTT ATG GTA GAG CCC GCC TGG G |
The 5′ primer was GGG GGA ATT CAT GGC ATC AGA GTT CAA AGA GAC CCC C.