FIG. 4.

Production of infectious virus in induced cell lines. (A) Virus in the supernatants of induced KSHV-infected TIME, 293, CV-1, HFF, and SLK cells was concentrated by ultracentrifugation. Cells were induced by overexpression of RTA. Serial dilutions of the concentrated virus were then used to infect TIME cells. The infected TIME cells were stained for LANA at 48 hpi. The percentage of LANA-positive cells at each dilution for each cell type is shown. (B) Virus was harvested from the supernatants of induced TIME, 293, CV-1, HFF, and SLK cells infected in the presence or absence of 0.5 mM PFA. The percentage of LANA-positive cells for each cell type in the presence or absence of PFA is shown.