Skip to main content
. 2003 Jun;77(11):6138–6152. doi: 10.1128/JVI.77.11.6138-6152.2003

FIG. 6.

FIG. 6.

Productive infection of Δ32/Δ32 CCR5 PBMCs by HIV and SIV strains with CXCR4 blocked. Δ32/Δ32 CCR5 or wt/wt CCR5 PBMCs were preincubated for 1 h with medium alone or the inhibitor AMD3100, vMIP-I, or AMD3100 plus vMIP-I at 1,000 nM before addition of virus. (A) wt/wt CCR5 PBMCs were infected in triplicate with the R5 HIV-1 strain SF162. (B to G) Δ32/Δ32 CCR5 PBMCs were infected in triplicate with all other viruses (2044, HAN-2, P1019, TER, ETP, and SIVman4). All virus stocks had titers of between 104 and 105 FFU/ml on NP2/CD4/CCR5 or NP2/CD4/CXCR4 indicator cell lines. Supernatant was removed every 3 days up to day 18, and virus production was measured by RT enzyme-linked immunosorbent assay. All points represent the average for triplicate samples, and infections are representative of at least two independent infections.