TABLE 2.

Calculation defining the minimal number of RSV P115S IN dimers required for maximum full-site integration activity^a

Initial IN concn (nM)	Time of maximum activity prior to inactivation (min)	% Active IN leftb	Calculated IN left (nM)	IN dimers per viral endc
4	1	67	2.7	7.2
6	3	29	1.8	4.8
8d	3	29	2.4	6.4
12	3	29	3.5	9.3

^aThe maximum full-site integration activity was determined from Fig. 4 and 5. It was defined as the last time point showing maximum full-site activity. The average t_1/2 for full-site integration was 1.7 min, as defined in Table 1.

^bThe formula used to calculate the % active IN left (N) as a function of time is N = N₀e^−λt.

^cCalculated number of IN dimers per viral DNA end at maximum full-site integration activity at the chosen time of 1 or 3 min. For illustration, at 3 nM IN, the calculated number of IN dimers per end is 8 (4.5-kbp double-ended LTR substrate).

^dAverage of four experiments.