TABLE 3.
Comparison of full and half-site integration activities for wt RSV IN and RSV IN with mutations at position W233a
| IN variant | Concn (nM) | Full-site activity | Half-site activity |
|---|---|---|---|
| wt | 5 | 16.7 | 2.7 |
| 10 | 11.0 | 5.4 | |
| W233F | 5 | 16.0 | 5.3 |
| 10 | 9.8 | 6.5 | |
| W233E | 5 | 1.9 | 0.2 |
| 10 | 1.8 | 0.2 | |
| W233A | 5 | 0 | 0 |
| 10 | 0 | 0 |
a
The values for activity represent the percentage of donor incorporated into target DNA for full and half-site integration products produced under standard reaction conditions. The DNase I footprints associated with these activities are shown in Fig. 8A.