Figure 5.

Depletion of LTA results in the reduction of PMN-activating capacity of S. aureus supernatants. A) LTA was depleted from flucloxacillin-exposed (20 × MIC, FLU20) S. aureus supernatants as described in Materials and Methods. LTA content was measured before and after LTA-depletion using an ELISA. Data are expressed as mean ± SD of three experiments. *Asterisk indicates significant difference (transformation log₁₀(x + 0.5), ANOVA, Dunnetts post test). B) Neutrophils were incubated at 5 × 10⁶/ml overnight in medium alone, with the LTA-containing or the LTA-depleted bacterial supernatants. The IL-8 content in the PMN supernatants was measured using ELISA. Data are expressed as mean ± SD of three experiments. Significant difference was analyzed after a transformation log₁₀(x + 0.5) by ANOVA and Tukey-Kramer post test.