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. 2000 Jul;2(4):365–377. doi: 10.1038/sj.neo.7900103

Figure 1.

Figure 1

(A) Met expression and activation in DA3 cells. DA3 cell extracts were IP using SP260 antibody (1) or IP using SP260 antibody in the presence of immunizing peptide (2) and WB with SP260 antibody. Untreated DA3 cells (3) or DA3 cells treated with HGF/SF (1.25ng/ml) for 5 minutes (4) were lysed in the presence of sodium orthovanadate, cell extracts were IP with SP260 antibody and immunoblotted with anti-phosphotyrosine antibody (4G10). Met is expressed in DA3N cells and is rapidly phosphorylated in response to HGF/SF treatment. (B) HGF/SF induces membrane ruffling and scattering of DA3 mammary cells. CLSM DIC images of untreated (1–3) and HGF/SF-treated cells (20 ng/ml) (4–6) after 10 minutes (4,1) and 10 minutes+0.05 seconds (5, 2) and 0.1 second (6,3). Arrows indicate areas of ruffling (original magnification, x 450).