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. 2000 Jul;2(4):339–345. doi: 10.1038/sj.neo.7900105

Figure 5.

Figure 5

FACS analyses of the parental clone X-21 and bcl-2-transfected X-21 cells showing percentage of cells that were subdiploid in parental untreated control, ara-C-treated (1.0 µg/ml for 72 hours), bcl-2-transfected X-21 as control, bcl-2-transfected cells treated with ara-C (1.0 µg/ml for 72 hours), parental clone X-21 cells pretreated with BACMK and zVAD (two caspase inhibitors), for 30 minutes and then exposed to ara-C (1.0 µg/ml) for 72 hours. Note the presence of approximately 27% of cells showing apoptosis in X-21 cells treated with ara-C. However, there was no significant increase in the frequency of apoptotic cells in bcl-2-transfected cells treated with ara-C as compared to the control. Caspase inhibitor treatment before ara-C exposure protected X-21 cells by 12% to 13% (A). Telomeric area measurements in percentages of the parental X-21 cells treated with ara-C (1.0 µg/ml for 72 hours), bcl-2-transfected X-21 cells (control), bcl-2-transfected X-21 cells-treated with ara-C (1.0 µg/ml for 72 hours), BACMK- and zVAD-pretreated X-21 cells exposed to ara-C (1.0 µg/ml for 72 hours). The percent telomeric area in interphase nuclei of FISH preparations was quantitated as compared to the total nuclear areas and expressed as the mean value (B). These values are just the reverse of values shown in (A).