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. 2000 Jul;2(4):315–324. doi: 10.1038/sj.neo.7900098

Figure 5.

Figure 5

The expression patterns of mRNAs for MMPs, u-PA and u-PAR in PC-14, A549 and Lu-99. PCR was performed by using specific primers for the MMPs, u-PA and u-PAR. The mRNA-derived PCR products for MT1-MMP (550 bp), MMP-1 (646 bp), MMP-2 (480 bp), MMP-3 (566 bp), MMP-7 (920 bp), MMP-9 (361 bp), u-PA (474 bp) and u-PAR (455 bp) were amplified for 30, 30, 30, 33, 30, 36, 30 and 30 cycles for in vitro cultured cells, or for 30, 30, 30, 30, 24, 39, 27 and 30 cycles for in vivo tumor tissues, respectively. The PCR products were separated on 1.5% agarose gels. The intensity of the RT-PCR products for each of the MMPs, u-PA and u-PAR was determined by densitometric analysis and normalized by the intensity of the PCR product derived from GAPDH mRNA. PC-14 (closed columns); A549 (hatched columns); Lu-99 (open columns).