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Comparison of MT1-MMP and MMP-2 mRNA expression in PC-14, A549 and Lu-99. RT-PCR products for MT1-MMP (550 bp), MMP-2 (480 bp) and GAPDH (983 bp) were amplified for 30, 30 and 21 cycles for in vitro cultured cells, or for 30, 30, and 24 cycles for in vivo tumor tissues, respectively. The amplified products were separated on 1.5% agarose gels.
